RNA is not only an essential intermediate in the flux of genetic information from DNA to proteins, but rather a molecule that plays crucial roles in the regulation of fundamental cellular processes. Importantly, the dysregulation of certain RNAs has been shown to be implicated in numerous pathological processes, including cancer. The transcriptome is reversibly and dynamically regulated by chemical modifications, adding a new layer of complexity and functionality to the emerging roles of RNAs in health and disease. The impact of these modifications has recently begun to be explored within a new field of study: ‘Epitranscriptomics’. Providing a new level of knowledge on the interplay between epigenetic and RNA modifications is a requisite for the development of novel promising therapeutic compounds for use in breast cancer patients.
Strengths in lab (technologies, methods):
We combine classical biochemical methods with state-of-the-art genome-wide sequencing and proteomic techniques to interrogate the role of RNAs modifications and the interplay with other epigenetic marks in stem and cancer cells. 2D and 3D cell culture models, patient samples, as well as mouse orthotropic transplantation are also used to study the central RNA-based regulatory circuitry in breast tumorigenesis.
The AguiloLab is part of the Wallenberg Centre for Molecular Medicine. Our research is made possible by funding from the Knut and Alice Wallenberg Foundation, Umeå University, Kempe foundation, Cancerforskningsfonden Norrlandand, and the Swedish Research Council.